Masliah Lab, University of
California, San Diego
Standard Operating Procedures:
Diaminobenzidine
#1 Process:
Use of
Diaminobenzidine (DAB) as a substrate in
immunocytochemistry techniques.
#2 Hazardous Chemicals/Class of Hazardous
Chemicals:
Diaminobenzidine, benzidine, Class: Carcinogen.
#3 Personal Protective Equipment:
a) latex
gloves, lab coats, eye protection (goggles) are to be worn during all
procedures involving DAB.
#4 Engineering/Ventilation Controls:
All
diluting and aliquoting of DAB solutions will be carried out inside of the fume
hood.
#5 Special Handling Procedures and Storage
Requirements:
a) - DAB
powder is stored in -20 oC freezer (25 gram vials).
-
Stock solutions (10 mg/ml) in 500 ul aliquots are stored at -20 oC
b) - The
weighing out of powdered DAB, mixing of stock solutions, and all aliquoting of
stock will be performed solely by Dr. Masliah (or Anthony Adame).
- The
lab will be cleared of all personnel during the weighing, mixing, and
aliquoting of DAB stock.
#6 Spill and Accident Procedures:
Currently
under evaluation (March 2003)
#7 Waste Disposal:
Bag and tag as hazardous chemical waste. Call
EH&S for disposal.
#8 Approval Required:
Lab personnel using DAB are required to obtain
prior approved of the P.I prior to working with DAB.